Chemical Control of a CRISPR-Cas9 Acetyltransferase
نویسندگان
چکیده
منابع مشابه
Chemical Control of a CRISPR-Cas9 Acetyltransferase.
Lysine acetyltransferases (KATs) play a critical role in the regulation of transcription and other genomic functions. However, a persistent challenge is the development of assays capable of defining KAT activity directly in living cells. Toward this goal, here we report the application of a previously reported dCas9-p300 fusion as a transcriptional reporter of KAT activity. First, we benchmark ...
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HYMS Centre for Education Development (CED), Hull York Medical School, University of York, York, UK Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK Department of Haematology, Oxford University NHS Foundation Trust, Churchill Hospital, Oxford, UK Academic Unit of Child Health, Sheffield Children’s Hospital, She...
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Prokaryotes use diverse strategies to improve fitness in the face of different environmental threats and stresses, including those posed by mobile genetic elements (e.g., bacteriophages and plasmids). To defend against these elements, many bacteria and archaea use elegant, RNA-directed, nucleic acid-targeting adaptive restriction machineries called CRISPR -: Cas (CRISPR-associated) systems. Whi...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2018
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.7b00883